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Product Name
GAPDH Mouse mAb (HRP)
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Gene ID
2597
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SwissProt ID
P04406
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Gene Name
GAPDH
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Alternative Names
G3PD; GAPD; HEL-S-162eP
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Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
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Research Field
Neuroscience
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Product Categories
Loading Control antibody
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Host
Mouse
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Reactivity
Human,Mouse,Rat
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Application
WB,ELISA
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Cited
WB (Monkey)
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Dilution Ratio
WB: 1/1000-1/5000 ELISA: 1/10000
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Molecular Weight
Calculated MW: 36 kDa; Observed MW: 36 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
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Isotype
IgG1
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Immunogen
A synthetic peptide corresponding to residues near the amino terminus of human GAPDH protein.
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Purification
Affinity Purified
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Conjugation
HRP
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C for at least 6 months. Avoid freeze/thaw cycles